植物生理學實驗實訓綜合教程（中英雙語版）

中文篇
 
第1章 植物生理學實驗基礎 3
第2章 植物種子的萌發(fā) 14
第3章 植物水分代謝 22
第4章 礦質(zhì)元素缺乏對植物生命活動的影響 30
第5章 植物光合性能的評價 42
第6章 植物碳氮代謝 59
第7章 植物生長物質(zhì)的生理效應及其對植物生長發(fā)育的影響 72
第8章 植物組織培養(yǎng) 83
第9章 植物逆境生理 95
 
附　錄 115
附錄1 相關理論知識鞏固與思考115
附錄2 化學品標志符號202
附錄3 常用酸堿的濃度203
附錄4 常用固態(tài)酸、堿、鹽的物質(zhì)的量濃度配制參考 203
附錄5 常用緩沖液的配制204
附錄6 常用酸堿指示劑209
附錄7 標準計量單位210
附錄8 常用植物生長物質(zhì)的一些化學特性212
參考文獻 213
 
英文篇
Chapter 1 Experimental Basis of Plant Physiology 217
 
11 Plant physiology laboratory rules217
 
12 Classification and preparation methods of chemical reagent218
 
13 Collection, processing and preservation of experimental material220
 
14 Basic methods of experimental studies on plant physiology228
 
Chapter 2 Plant Seed Germination 232
 
21　 Determination of seed germination rate, germination potential and 
germination index233
 
22　 Rapid determination of seed viability234
 
23　 Determination of seed vigor236
 
24　 The formation and determination of the amylase activity during seed 
germination238
 
25　 Changes in fatty acid content of oil seeds during germination239
 
26　 Changes in amino acid content during seed germination241
 
Chapter 3　 Plant Water Metabolism 243
 
31　 Determination of plant leaf water content, relative water content and 
water saturation deficit244
 
 
 
32　 Determination of free and bound water content in plant leaves 
(Malincic method) 245
 
33　 Determination of plant tissue water potential (Small flow method) 247
 
34　 Determination of plant tissue osmotic potential (Plasma wall 
separation method) 249
 
35　 Determination of transpiration rate251
 
Chapter 4　 Effects of Mineral Element Deficiency on Plant Life 
Activities 254
 
41　 Element deficiency symptoms in plants (solution culture) 255
 
42　 Determination of nitrate reductase activity259
 
43　 Determination of root activity (α - naphthylamine oxidation method) 261
 
44　 Determination of nitrate nitrogen in plants263
 
45　 Determination of plant phosphorus (molybdenum blue method) 265
 
Chapter 5　 Evaluation of Plant Photosynthetic Performance 268
 
51　 Extraction and physicochemical properties of photosynthetic pigments269
 
52　 Separation of photosynthetic pigments and determination of absorption 
spectra271
 
53　 Determination of chloroplast pigment content273
 
54　 Determination of plant leaf photosynthetic rate and gas exchange parameters
276
 
55　 Determination of chlorophyll fluorescence parameters280
 
56　 Determination of ribulose diphosphate carboxylase (RuBPCase) 
activity286
 
57　 Determination of phosphoenolpyruvate carboxylase (PEPCase) 
activity289
 
 
 
Chapter 6　 Plant Carbon and Nitrogen Metabolism 291
 
61　 Determination of sucrose, glucose and fructose content292
 
62　 Determination of free amino acid content294
 
63　 Determination of sucrose synthase and sucrose phosphatase activities296
 
64　 Determination of α - amylase and β - amylase activities298
 
65　 Determination of glutamine synthetase activity302
 
66　 Determination of soluble protein content304
 
Chapter 7　 The Physiological Effects of Plant Growth Substances and 
Their Effects on Plant Development 307
 
71　 Effects of auxin on the growth of wheat root and shoot308
 
72　 Effects of gibberellin on the growth of wheat seedlings310
 
73　 Effect of ethephon on the fruit ripening310
 
74　 Abscissive effects of abscisic acid (ABA) on plant petioles311
 
75　 Effects of gibberellin (GA3) and abscisic acid (ABA) on seed 
germination313
 
76　 Synthesis of α - amylase in seed induced by gibberellin (GA3) 314
 
77　 Effects of abscisic acid (ABA) on stomatal movement317
 
78　 Determination of phytohormones by liquid chromatography318
 
Chapter 8　 Plant Tissue Culture 320
 
81　 Preparation of culture medium321
 
82　 Sterilization, disinfection and inoculation325
 
83　 Subculture and propagation330
 
84　 Acclimation, transplanting and management of tube seedlings334
 
Chapter 9　 Plant Stress Physiology 336
 
91　 Determination of proline content337
 
92　 Determination of total flavonoid content339
 
 
 
93　 Determination of ascorbic acid (AsA, AA) 341
 
94　 Determination of glutathione (GsH) 345
 
95　 Determination of cytoplasmic membrane permeability - conductance 
method347
 
96　 Identification of degree of membrance peroxidation - determination of 
malondialdehyde content (MDA)349
 
97　 Identification of cell death in plant tissues (Trypan blue staining method) 351
 
98　 Determination of hydrogen peroxide (H2O2) content352
 
99　 Determination of superoxide anion production rate354
 
910　 Determination of superoxide dismutase (SOD) activity356
 
911　 Determination of peroxidase (POD) activity358
 
912　 Determination of catalase (CAT) activity360